Sample preparation

CNH concentration and stable isotopes of solid samples

Note: Before use clean utensils using ethanol and Kimwipes, allow to dry!

Sediment, soil and organic material
Note:  Carbonatic sediments need to be analyzed using two preparation pathways for CNtot and Corg!

Sample Quantities:
Organic materials 1–2mg                    
Sediment 5–40mg

CNtot analysis:
Samples should be dried/freeze-dried and homogenized.                    
Weigh a quantity of material in tin capsules 5x8mm (1–20mg) or silver capsule 6x6x12mm
(more than 20mg), seal  and place the samples into a sample tray.                    
Please provide samples and a file with location, sample name and weight.

Corg analysis:
Samples should be dried/freeze-dried and homogenized.                    
Weigh a quantity of material in silver capsules 6x6x12mm and add a few drops of 2N HCl (should cover all material).                    
If reaction occurs, i.e. bubbles are created, dry the sample for 1h at 60 degrees and repeat the addition of acid.    
Dry overnight at 60 degrees.
Seal the capsule and place them in a sample tray.

Please provide samples and a file with location, sample name and weight.

GFF filter samples
Corg, N analysis:

Add the wet filters in a desiccator with conc. HCl overnight.                    
Dry them in a desiccator with bluegel at 60 degree overnight.                    
Pack them in silver boats 6x6x12mm and put them in a sample tray.                
Please provide samples and a file with location, sample name and perhaps filtered volumes.

 

S concentration and stable isotopes of solid samples

Note: Before use clean utensils using ethanol and Kimwipes, allow to dry!

Sediment, soil, organic materials and prepared S samples (BaSO4, etc.)

Sample Quantities:  
sample type weight
BaSO4 0.5mg
FeS2 0.2mg
PbS 0.5mg
ZnS 0.2mg
Ag2S 0.5mg
organic materials, dependence of S content 2–10mg

S analysis:
Samples should be dried/freeze-dried and homogenized.                    
Weigh a quantity of material in tin capsules 8x5mm and add the same amount of V2O5 (should be 1:1 but max 2mg, shake a bit so that the sample material and V2O5 are mixed).    
Seal the capsule and place them in a sample tray.                    
Please provide samples and a file with location, sample name and weight.

 

O stable isotopes of solid samples

Note: Before use clean utensils using ethanol and Kimwipes, allow to dry!

Note: For better precision each sample should be measured twice!                   

Phosphate
Samples should be dried/freeze-dried.                    
Weigh 0.5 mg of silver phosphate in silver capsules 3.5x5mm and place them in a sample tray.    
Before analysis, dry weighed samples overnight at 60 degrees.                    
Please provide samples and a file with location, sample number and weight.

Sulfate
Samples should be dried/freeze-dried.                    
Weigh 1 mg of barium sulfate in silver capsules 3.5x5mm and place them in a sample tray.    
Before analysis, dry weighed samples overnight at 60 degrees.                    
Please provide samples and a file with location, sample number and weight.

Cellulose
Samples should be dried/freeze-dried.                    
Weigh 2mg materials in silver capsules 3.5x5mm and place them in a sample tray.
Before analysis, dry weighed samples overnight at 60 degrees.                    
Please provide samples and a file with location, sample number and weight.

 

H stable isotopes of solid samples

Note: Before use clean utensils using methanol!! and Kimwipes, allow to dry!

Organic material
Samples should be dried at 60 degrees for 24h and grounded to a fine powder.
Weigh 0.35mg ±0.020mg material in silver capsules 3.5x5mm and place them in a sample tray.
Please provide samples and a file with location, sample number and weight.

 

OH stable isotopes of water samples

Water samples
The samples should be stored cool between sampling and analysis.                
Fill the vials (2 ml) so that there is a small air bubble.                    
Close them (blue screw cap, PTFE/SILICONE/PTFE septum).                    
Please provide samples and a file with sample name.

 

C stable isotopes of DIC

Water samples
Add 100ul of concentrated phosphoric acid in the vials.                
Close the vials with a white cap/septum (the acid should not come in contact with the septum).    
Flush the vials with Helium (100ml/min) for 4 minutes. The prepared vials could be used several weeks.
Inject max 5ml sample of water through the septum.                   
Note! Samples should be injected into the vial during the sampling in the field (outgassing of CO2!).
The samples should be stored cool between sampling and analysis. Before analysis put the samples at room temperature over night.

Please provide samples and a file with sample number and name.

 

C stable isotopes of DOC

Water samples
 link to PDF article

Please provide samples and a file with sample number and name. 

CO stable isotopes of carbonate

Note: Before use clean utensils using ethanol and Kimwipes, allow to dry!

Carbonate
Samples should be dried/freeze-dried and homogenized.                    
Mark glass vials (Labco 738W – white cap).                    
Weigh 0.2mg  sample material/standard in the vials (applies to 100% carbonate samples).    
Use a silver cap and check after each sample the weight!                    
Close the vials with caps/septa.

Dry overnight with removable lid at 90 degrees and if foraminifere dry at 50 degrees.

Take out and add in a horizontal position 100ul conc H3PO4 on the wall (acid shouldn't touch the sample).

Close the lid and put the vial in the flush rack still horizontal.

Flush the vials with Helium (100ml/min) for 10 minutes.

Place all tubes in the vertical position and allow acid to react at room temperature with the samples overnight. 

If the samples are not calcite the temperature and time for the reaction may differ.

Please provide samples and a file with sample number and namn.

 

CH stable isotopes of methane

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